저온순화 및 탈순화가 배추의 내동성 및 total RNA, soluble protein, soluble sugar 함량에 미치는 영향

초록

This study was conducted to determine the change in freezing tolerance of Chinese cabage (Brassicacampestris L. ssp. penkinensis). Four-week old seedlings grown in a greenhouse at 25oC0.5 weretransferred to 5, 10 or 15oC condition for acclimation for one day, and then transferred to a lethaltemperature or -3oC condition. Optimal acclimation temperatures were 5oC and 10oC. Freezing toler-ance of leaf tissues was investigated during a week-long cold stress. Development of freezing tolerancewas shown by survival ratio, and amounts of total RNA, soluble protein and soluble sugar. LT50 valueincreased from -3 to -4oC after 5 days acclimation at 5 or 10oC, and this was considered to be the firstindication of enhanced freezing tolerance. Plants, cold acclimated for five days, reached to a survivalratio of 60%. This increase in freezing tolerance was found to be associated with the increased levelsof total RNA, soluble sugar and soluble protein. These metabolic changes imply the association ofadjustment of growth and cell metabolism with low temperatures at the beginning of cold acclimationin Chinese cabbage.

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