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Functional Expression of Amylosucrase, a Glucan-Synthesizing Enzyme, from Arthrobacter chlorophenolicus A6
- Seo, Dong-Ho;
- Jung, Jong-Hyun;
- Choi, Hyun-Chang;
- Cho, Hyun-Kuk;
- Kim, Hee-Hang;
- ... Ha, Suk-Jin;
- 외 3명
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28초록
A gene (acas) designated as alpha-amylase was cloned from Arthrobacter chlorophenolicus A6. The multiple amino acid sequence analysis and functional expression of acas revealed that this gene really encoded an amylosucrase (ASase) instead of alpha-amylase. In fact, the recombinant enzyme exhibited typical ASase activity by showing both sucrose hydrolysis and glucosyltransferase activities. The purified enzyme has a molecular mass of 72 kDa and exhibits optimal hydrolysis activity at 45 degrees C and a pH of 8.0. The analysis of the oligomeric state of ACAS with gel permeation chromatography revealed that the ACAS existed as a monomer.
키워드
Amylosucrase; Arthrobacter chlorophenolicus; glucan-synthesizing enzyme; sucrose; RECOMBINANT AMYLOSUCRASE; SUCROSE; GENE
- 제목
- Functional Expression of Amylosucrase, a Glucan-Synthesizing Enzyme, from Arthrobacter chlorophenolicus A6
- 저자
- Seo, Dong-Ho; Jung, Jong-Hyun; Choi, Hyun-Chang; Cho, Hyun-Kuk; Kim, Hee-Hang; Ha, Suk-Jin; Yoo, Sang-Ho; Cha, Jaeho; Park, Cheon-Seok
- 발행일
- 2012-09
- 유형
- Article
- 권
- 22
- 호
- 9
- 페이지
- 1253 ~ 1257
- 언어
- ENG
- 출판사
- KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY
- 발행국가
- 대한민국
- 분량
- 5 페이지
- ISSN
- E 1738-8872
P 1017-7825