대식세포주 RAW264.7 세포에서 Curcumin의 Lipopolysaccharide 에 의한 Nitric Oxide 생성 억제 효과

초록

ABSTRACT : Curcumin, a polyphenolic antioxidant purified from turmeric, has been known to possess various biological activities such as anti-oxidative, anti-inflammatory and anti-cancer effects. In this study, we have explored anti-inflammatory effect of curcumin using Gram (−) bacterium-derived endotoxin (lipopolysaccharide: LPS) and macrophage cell line RAW264.7. Curcumin suppressed NO production in LPS-activated RAW264.7 cells in a dose-dependent manner. Curcumin also blocked the activation of NF-κB but not AP-1 according to luciferase assay. Furthermore, this compound suppressed the phosphorylation of a series of intracellular signaling components such as Src, JAK-2, Akt, IKK and IκBα under LPS stimulation in a time dependent manner. Therefore, our data suggest that curcumin was able to protect the host from Gram (−) bacterial-infection-mediated inflammatory symptoms.

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