Reference proteome map of buckwheat (Fagopyrum esculentum and Fagopyrum tataricum) leaf and stem cultured under light or dark

초록

Buckwheat sprouts are a vegetable to provide health benefit with their nutritionally important substances. Despite several reports on describing buckwheat as preventive medicine, little has been studied on the developmental physiology of buckwheat in protein level. Thus, we attempted for the first time to examine the proteomic profiles of leaf and stem from 7-day-old etiolated sprouts of common (Fagopyrum esculentum Moench.) and tatary buckwheat (Fagopyrum tataricum Gaertn.) seedlings under light and dark. By the gel-based proteomic approach, 166 unique proteins were identified and six proteins were commonly found from both buckwheat species in which 79 and 81 proteins were exclusively belonged to common and tatary buckwheat, respectively. The most abundant proteins were assigned to metabolism and cytoplasmic proteins were dominated by the classification of molecular function and intracellular localization. The sprouting leaf of 7-day-old etiolated common buckwheat seedlings showed light yellow under dark, suggesting the inhibition of light-dependent protochlorophyllide reductase leading to the reduction of chlorophyll biosynthesis. Light-inhibited storage protein, 13S globulin 3, was commonly found in the leaves of both buckwheat species under dark. In particular, the unknown pentatricopeptide repeat-containing proteins were identified in light-induced leaf of common and tatary buckwheat and also identified in dark-induced stem of tatary buckwheat. Thus, it suggests that the different and specific regulators are involved in the sterility and fertility from common and tatary buckwheat in a specific light-dependent manner. The reference proteomic map of buckwheat will give insight for understanding buckwheat physiology and application to buckwheat industry.

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