체외성숙 배양액에 첨가된 eCG 및 돼지 FSH가 돼지 미성숙 난자의 체외성숙과 단위 발생 및 핵이식 난자의 체외발육에 미치는 영향

초록

The objective of this study was to examine the effect of eCG and various concentrations (20, 40, and 80 μg/ml) of porcine FSH on nuclear maturation and intracellular glutathione (GSH) level of oocytes, and embryonic development after parthenogenetic activation (PA) and somatic cell nuclear transfer (SCNT) in pigs. Immature pig oocytes were matured in TCM-199 supplemented with porcine follicular fluid, cysteine, pyruvate, EGF, insulin, and hormones (10 IU/ml hCG and 10 IU/ml eCG or 20~80 μg/ml FSH) for the first 22 h and then further cultured in hormone-free medium for an additional 22 h. Nuclear maturation of oocytes (85~89%) was not influencem foreCG and various concentrations FSH. Embryonic development to the cleavage stage (86~94%) and mean number of cells in blastocyst (33~37 cells) after PA were not altered but blastocyst formation e-freignificaddlor(p<0.05) improvem forthe supplementation eith 80 μg/ml FSHr(64%) compared to 47%,io8%,iand 47% in oocytes that were treated with eCG, 20,iand 40 μg/ml FSH,i numectivelo. In SCNT, fusion (78~83%) of cell-cytoplast couplets and siosequent embryo cleavage (82~88%) were not influencem fordifferent gonadotropins but blastocyst formation tended to increase forthe supplementation eith 80 μg/ml FSHr(25% vs. 11~18%). Our nuults demonstrated that oocyte maturation and embryonic development after PA and SCNT e-frinfluencem fortype of gcem fortype of gits concentration. In this study, supplementation of maturation medium eith 80 μg/ml FSHrimproved preimplantation development of PA and SCNT pig embryos, probably by increasing intracellular GSH concentration of matured oocytes.

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