High catalytic activity of gold nanoparticle-templated, tyrosine-rich peptide self-assemblies for 3,3′,5,5′-tetramethylbenzidine oxidation in the absence of hydrogen peroxide

초록

In this study, gold nanoparticle-templated, tyrosine-rich peptide (YC7@AuNPs) self-assemblies were examined as peroxidase enzyme-mimic catalysts for the oxidation of 3,3 ',5,5 '-tetramethylbenzidine (TMB) and its activity was monitored by changing the reaction conditions such as pH, temperature, initial TMB concentration, and catalyst (Au) loading amount. The YC7@AuNPs catalysts were active for TMB oxidation even in the absence of hydrogen peroxide. Michaelis-Menten kinetic analysis showed that the YC7@AuNPs catalysts without hydrogen peroxide have activities comparable to other peroxidase-mimic catalysts using hydrogen peroxide in terms of the Michaelis constant.

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