Protein profile changes in platelet concentrates according to storage and leukoreduction - Analysis using proteomics technology

초록

Background: Knowing how the protein profile of platelet products changes with storage or leuko-reduction may give us greater insight into cell physiology and the cause of transfusion reaction other than cytokines and chemokines. Methods: We filtered four packs of platelet concentrates (PC) within 24 hr of blood collection and after 120 hrs of storage. Four aliquots of each supernatant in PC were obtained: pre-storage + prefilteration, pre-storage+post-filtration, post-storage+pre-filtration and post-storage-post-filtration. Routine chemistry tests and a two-dimensional electrophoresis (2-DE) were performed. The stained images were analyzed and the insignificant sports were identified using a peptide mass finger printing (PMF) with matrix assisted laser desorption/ionization-time of flight (MALDI-TOF) analysis after trypsion digestion. Results: The protein spots increased with storage and decreased after filtration (P < 0.05, pre-storage+post-filtration). The sport density of various proteins, including macrophage inflammatory protein-2 alpha, megakaryocyte colony stimulating factor and interleukin-22 changed with storage and leukoreduction. Conclusions: The database of identified protein spots and their changes produced in this study is a useful basic tool for future studies on the mechanism of transfusion reactions. Further studies should validate the significance of each protein spot.

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