Effects of the Constituents of Paeonia lactiflora Root on Arachidonate and NO Metabolism

초록

In order to establish the anti-inflamatory cellular mechanism of the paeony root (Paeonia lactiflora,Pall, Paeoniaceae), the constituents including paeoniflorin, albiflorin, (+)-catechin, paeonol, benzoic acid andmethyl gallate were evaluated for their effects on arachidonate and NO metabolism. Among the compoundstested, only paeonol weakly inhibited cyclooxygenase-2-mediated PGE2 production from LPS-treated RAW264.7 cells. (+)-Catechin and methyl galate weakly inhibited inducible nitric oxide synthase-mediated NO pro-cells with an IC50 of 8.4 M. These results suggest that the inhibition of these components on arachidonate andNO metabolism may contribute at least in part to anti-inflamatory mechanism of the paeony root. Keywords . Paeonia lactiflora, cyclooxygenase, nitric oxide synthase, lipoxygenase, methyl gallate

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