Cloning and morphological properties of Nicgl;CYCD3;1 gene in genetic tumors from interspecific hybrid of N. langsdorffii and N. glauca

초록

Plant genetic tumors represent neoplastic growths, which arise spontaneously in hybrid plants without apparent external induction. To understand the molecular nature of unregulated cell proliferation, a cyclin D cDNA clone encoding a cyclin D of 1104bp was isolated from a genetic tumor and designated Nicgl;CYCD3;1 gene. DNA get blot analysis suggested that there are two copies of Nicgl; CYCD3; I in the genetic tumors. Northern analysis showed that this gene had the highest expression level in genetic tumor compared to Nicotiona glauca, N. longsdorffii and hybrid plants. Plant morphology of hybrid plant was an intermediate between N. glauca and N. longsdorffii and was altered in the genetic tumors. The cell cycle distribution in N. glouca was G(0)/G(1), 90.59; S, 0.60; G(2)/M, 8.81; in N. langsdorffii it was G(0)/G(1), 86.22; S, 6.90; G(2)/M, 6.88; in hybrid plants it was G(0)/G(1), 96.40; S, 1.79; G(2)/M, 1.81; and in genetic tumors G(0)/G(1), 74.70; S, 2.35; G(2)/M, 22.94. These data provide new insights into the molecular mechanisms underlying genetic tumor formation from interspecific hybrid between N. langsdorffii and N. glouca. (C) 2007 Elsevier GmbH. All rights reserved.

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