Composition and Cytotoxicity of Essential Oil from Korean rhododendron (Rhododendon mucronulatum Turcz. var. ciliatum Nakai)

초록

The essential oil was obtained from the aerial part of Rhododendon mucronulatum Turcz. var. ciliatum Nakai by steam distillation, samples were collected by headspace (HS) and solid-phase microextraction (SPME) methods, and the compositions of the oil were analyzed by gas chromatography-mass spectrometry (GGMS). Nineteen constituents were identified from the essential oil: 15 carbohydrates, 3 alcohols, and 1 acetates. Major constituents were 2-beta-pinene (16.1%), camphene (11.9%), delta-3-carene (11.4%), d,l-limonene (9.5%), and gamma-terpinene (9.5%). By SPME extraction, seventeen constituents were identified: 13 hydrocarbons, 1 alcohol, 1 nitrogen-containing compound, 1 acetate, and 1 amine. Major constituents of the SPME-extracted sample were camphene (19.6%), 2-beta-pinene (18.0%), delta-3-carene (17.4%), trimethyl hydrazine (9.7%), gamma-terpinene (8.5%), and d,l-limonene (5.5%). By HS extraction, thirteen constituents were identified: 11 hydro-carbons, 1 alcohol, and 1 nitrogen-containing compound. Major constituents of the HS-extracted sample were camphene (25.8%), delta-3-carene (24.8%), 2-beta-pinene (20.2%), d,l-limonene (5.4%), tricyclene (5.1%) and trimethyl hydrazine (4.6%). The fragrance of the essential oil was coniferous, balsamic, and woody, and the IC50 value of the essential oil was 0.030 mu g/mg in MTT assay using HaCaT keratinocyte cell line.

키워드