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초록
A stereospecific HPLC method has been developed for the resolution of the enantiomers of salbutamol in human urine. After solid-phase extraction and derivatization with (S)-(-)-α-methyl-benzyl isocyanate, the diastereomeric derivatives were resolved (R<inf>s</inf>=1.59) on 5 μm octadecylsilan column using 47% methanol as a mobile phase with fluorescence detection. The detection limit of each enantiomer was 10 ng/ml (S/N=3).
키워드
(S)-(-)-α-methylbenzyl isocyanate; Chiral derivatizing reagent; Chiral separation; Enantiomer; HPLC; Salbutamol
- 제목
- Resolution of salbutamol enantiomers in human urine by reversed-phase high performance liquid chromatography after derivatization with (S)-(-)-α-methylbenzyl isocyanate
- 저자
- Kim, Kyeong-ho; Kim, Tae-kyun; Kwon, Young-hee; Sohn, Young-taek
- 발행일
- 1997
- 유형
- Article
- 권
- 20
- 호
- 5
- 페이지
- 486 ~ 490