Assessment of Bacteriophage-induced Inflammatory Mediators in Salmonella-infected Chicken Macrophage HD11 Cells

초록

This study was designed to characterize the role of P22 in the intracellular survival and pro-inflammatory responses in Salmonella-infected chicken macrophage-like HD11 cells. The intracellular survival of Salmonella Typhimurium was evaluated in the presence or absence of P22 at 24h postinfection (hpi). The production of inflammatory mediators, including nitric oxide (NO), IL-1 beta, IL-6, IL-8, and IL-10, was determined at 0 and 24 hpi by using ELISA kits. The survival rates of intracellular S. Typhimurium were effectively reduced to 8.1% in Salmonella-infected HD11 cells treated with P22 at 24 hpi. The secretion of NO, IL-6, IL-8, and IL-10 varied with infection period and bacteriophage treatment. The reduction of intracellular S. Typhimurium was highly correlated with the enhanced production of NO (r=0.816, P<0.001). The results suggest that bacteriophage can be used to control the intracellular pathogens. This study would provide useful information on the bacteriophage-host interactions and open the door for designing an effective and safe bacteriophage therapeutic system.

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