미니돼지 정액 보존에 있어서 해양성 광천수의 영향

초록

The objective of this study was to evaluate the sperm liquid storage diluted with Brine Mineral Water (BMW) in miniature pig. Therefore we performed to find optimal concentration of BMW. The ejaculated semen from miniature pig was collected by gloved-hand method. The collected semen was diluted with dilution solution (Mulberry Ⅲ; modified-Modena B) which BMW was added. Concentration of BMW was 0, 2.5, 5, 7.5, 10 and 12.5% in dilution solution. The diluted semen was preserved at 17℃. Sperm ability was estimated by viability, capacitation acrosome reaction using chlortetracycline (CTC), hypoosmotic swelling test (HOST), morphologic abnormality. The diluted semen was observed for 7 days. The viability was significantly measured higher at 2.5% concentration of BMW than other groups (p<0.05). The AR-pattern of CTC analysis was significantly measured lower at 12.5% concentration of BMW than other groups (p<0.05). However, abnormality was not significantly different among all the groups (p<0.05). In conclusion, viability of sperm was the highest at 2.5% concentration of BMW but BMW had a negative effect on HOST, capacitation and acrosome reaction in sperm of miniature pig.

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