독일가문비(Picea abies Karsten) 잎 추출성분의 항산화 활성

초록

The dried needles (1.5 kg) of Picea abies Karsten were ground, extracted with acetone-H2O (7:3, v/v), concentrated, and fractionated with a series of nseparatory funel. Each fraction was freeze dried, then a portion of ethyl acetate soluble powder waschromatographed on a Sephadex LH-20 column using a series of aqueous methanol and ethanol-n-hexanemixture as eluents. The isolated compounds were identified by cellulose TLC, 1H-, 13C-NMR, FAB and EI-MS.(+)-catechin (compound I), ()-epicatechin (compound II), kaempferol-3-O-β-D-glucopyranoside (compound III),4-hydroxyacetophenone (compound IV) were isolated from the ethyl acetate soluble fraction and (+)-catechin(compound I), protocatechuic acid (compound V) were isolated from the H2O soluble fraction of P. abies needle.The antioxidative activities of each fraction and the isolated compounds were tested by DPPH radicalscavenging method, and EtOAc soluble fraction, (+)-catechin and ()-epicatechin showed similar values to α-tocopherol and BHT as controls.

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