Effect of Bioprocessed Black Rice Bran on Muscle Strength in a Dexamethasone-Induced Muscle Atrophy C2C12 Model; 덱사메타손에 의한 근위축 유도 시험관 모델에서 흑미강 발효 추출물의 근력 개선 효과

초록

Muscle atrophy caused by aging, obesity, and inflammation has become a prominent global concern, prompting increased interest in developing functional food ingredients to address this issue. This study investigated the efficacy of bioprocessed black rice bran extract, a by-product of black rice processing, on enhancing muscle strength by treating a dexamethasone-induced muscle atrophy model during the differentiation of C2C12 myotubes with bioprocessed black rice bran (BRB-F-S). To evaluate the cytotoxicity of BRB-F-S, cell viability was measured using the 2,3-bis-(2-methoxy-4-nitro-5-sulphenyl)-(2H)-tetrazolium-5-carboxanilide (XTT) assay. Additionally, the cytoprotective effects of BRB-F-S against dexamethasone-induced muscle atrophy were quantitatively assessed via relative cell viability and myotube formation. The study results indicated that the BRB-F-S-treated group not only provided statistically significant protection against dexamethasone-induced cytotoxicity but also improved myotube formation. Furthermore, to investigate the mechanisms underlying the effects of BRB-F-S on myotube formation, an analysis of protein expression was carried out which revealed a significant increase in the levels of sirtuin 1, AMP-activated protein kinase alpha, protein kinase B, and mammalian target of rapamycin, which are proteins associated with mitochondrial biogenesis and protein synthesis, while expression levels of forkhead box class O 3a, atrogin1, and muscle RING-finger protein-1, related to protein degradation and the ubiquitin-proteasome system, were significantly reduced. These findings suggest that BRB-F-S could serve as a potential ingredient for health functional foods aimed at improving muscle atrophy. © 2025 The Korean Society of Food Science and Nutrition.

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