Genetic Diversity among Local Populationsof the Gold-spotted Pond Frog, Rana plancyi chosenica(Amphibia: Ranidae), Assessed by MitochondrialCytochrome b Gene and Control Region Sequences

초록

The Gold-spotted pond frog, Rana plancyi chosenica, designated as a vulnerable species by IUCN Red list.This species is a typical example facing local population threats and extinction due to human activities inSouth Korea. A strategic conservation plan for this endangered species is urgently needed. In order to provideinformation for future conservation planning, accurate information on the genetic diversity and taxonomic sta-tus is needed for the establishment of conservation units for this species. In this study, we used a moleculargenetic approach using the mitochondrial cytochrome b gene and control region sequences to find the geneticdiversity of gold-spotted pond frogs within South Korea. We sequenced the mitochondrial DNA cytochromeb gene and control region of 77 individuals from 11 populations in South Korea, and one from Chongqing,China. A total of 15 cytochrome b Korean gold-spotted pond frogs. Mean sequence diversity among Korean gold-spotted pond frogs was 0.31%(0.0-0.8%) and 0.51% (0.0-1.0%), respectively. Most Korean populations had at least one unique haplotypefor each locus. The Taean, Ansan and Cheongwon populations had no haplotypes shared with other popu-lations. There was a sequence divergence between Korean and Chinese gold-spotted pond frogs(1.3% for cytb band control region sequences indicate that the Korean gold-spotted pond frog are genetically differentiatedfrom those in China .

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