Characterization of starch-utilizing yeast Saccharomycopsis fibuligera Isolated from nuruk; Saccharomycopsis fibuligera

초록

A number of Saccharomycopsis fibuligera strains that can hydrolyse and utilize starch as a carbon source were isolated from nuruk, a traditional Korean starter for rice wine fermentation, and their specific growth rates on starch-containing medium were compared to choose the prominent strain. S. fibuligera strain MBY1320 showed a higher growth rate at 42°C than that of strain S fibuligera KCTC7806, indicating that S. fibuligera MBY1320 has more thermo-tolerant machinery for starch hydrolysis and utilization than KCTC7806 Although the activity of a-amylase at 30°C was significantly lower for S fibuligera MBY1320 than KCTC7806 (3,812.5 U vs. 14,878.5 U), S fibuligera MBY1320 showed a much higher glucoamylase activity at 42°C than S. fibuligera KCTC7806 (5,048.9 U vs 13,152 3 U). Thus, a new S. fibuligera strain, with a higher starch-hydrolysing activity at elevated temperatures than that of other types of strain, this study reports. © 2014, The Korean Society for Microbiology and Biotechnology.

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