Neuroprotective Activity of Androst-16-ene-3,6-diol Isolated from Lysimachia christinae

초록

In our prior research, we documented the robust neuroprotective activity of androst-16-ene-3,6-diol, which was extracted from the methanolic extract of Lysimachia christinae. This compound exhibited significant neuroprotection against glutamate-induced neurotoxicity in HT22 cells, which were subjected to injury caused by an excess of glutamate. In this study, our objective was to validate the neuroprotective effects of androst-16-ene-3,6-diol in HT22 cells subjected to glutamate-induced injury. Additionally, we aimed to elucidate the mechanisms underlying the neuroprotective activity of androst-16-ene-3,6-diol. Androst-16-ene-3,6-diol mitigated the elevated levels of reactive oxygen species induced by excessive glutamate treatment in HT22 cells. Furthermore, the treatment with androst-16-ene-3,6-diol led to a reduction in Ca2+ concentration. The compound also restored the mitochondrial membrane potential to its normal condition. Additionally, androst-16-ene-3,6-diol not only elevated the levels of glutathione reductase and peroxidase to those of the control but also increased the amount of glu-tathione, an endogenous antioxidant. These results suggested that androst-16-ene-3,6-diol isolated from L. christinae showed potent neuroprotective activity through the anti-oxidative pathway. © 2024, Korean Society of Pharmacognosy. All rights reserved.

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