Icariin 함량에 따른 삼지구엽초 추출물의 추출조건 및 항산화 효과

초록

study was conducted to determine the optimum condition of extraction and antioxidant activ-ity of ethanol extracts of Epimedium koreanum Nakai based on the icariin quantity. Also, further organic solvent frac-tions of hexane, chloroform, ethyl acetate and butanol were obtained from the ethanol extract of Epimedium koreanumNakai at different temperatures. Total ethanol extraction yield of wild grape seed ranged from 1.8% to 39.3% depend-ing on the concentration of icariin as well as diferent ethanol concentration, extraction temperature and time condi-tion. Among different extraction temperatures and time, the highest extraction yield of 39.3% was obtained at 70%ethanol for 3 hour at 50oC in the sample containing the e meantime, the strongest freeradical scavenging effect (RC50) with 18.9 g/mL in the KE9412 sample was observed in 70% ethanol extract of Epi-medium koreanum Nakai extracted for 7 hour at 70oC, while RC50 with 35.2 g/mL was observed in the KE9405(0.20% icariin content) sample at same condition. Also, antioxidant activity of ethanol extracts of Epimedium kore-anum Nakai increased as icarin concentration increased. Among each fraction obtained from organic solvents,butanol fraction was found to have the strongest RC50 (39.2 g/mL) and followed by ethylacetate (49.0 g/mL), water(118.8 g/mL), ethylacetate (119.8 g/mL), and chloroform fraction (138.5

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