Phylogenetic Relationship of Ligularia Species Based on RAPD and ITS Sequences Analyses

초록

The genetic relationships in 5 species of Ligularia were investigated using RAPD (Randomly Amplified Polymorphic DNA) and ITS (Internal Transcribed Spacer) sequences analyses. In RAPD analysis, sixty three of 196 arbitrary primers showed polymorphism. The amplified fragments ranged from 0.2 to 1.6 kb in size. The dendrogram was constructed by the UPGMA clustering algorithm based on genetic similarity of RAPD markers. A total of 16 accessions were classified into 5 major groups corresponding each species at the similarity coefficient value of 0.77. In the ITS sequence analysis, the size of ITS 1 was varied from 248 to 256 bp, while ITS 2 was varied from 220 to 222 bp. The 5.8S coding region was 164 bp in lengths. Forty nine sites (10.2%) of the 478 nucleotides were variable, and the G+C content of ITS region ranged from 49.4 to 53.5%. In the ITS tree, five species of Ligularia were monophyletic, and L. taquetii was the first branching within the clade. Ligularia intermedia formed a clade with L. fischeri var. spiciformis (BS=79), and L. stenocephala and L..fischeri were also claded. Two data sets were congruent, except of the position of L. fischeri var. spiciformis.

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