금나노입자-DNA Probe를 이용한 아프리카돼지열병(ASFV) 조기 진단 시스템

초록

African swine fever virus (ASFV) is a DNA virus epidemic disease that is known to have a fatality rate of close to 100% when infected. Because there is no vaccine for ASFV, early diagnosis that can be quickly checked in the field is very important. Also, it is known that the colloid solution has a colorimetric color because gold nanoparticles have a specific wavelength in the visible light region. When the wavelength is changed due to the aggregation or shape of the gold nanoparticles, the colorimetric of the solution is also changed. So, we use gold nanoparticles-DNA Probe-ASFV combination for efficient early diagnosis of ASFV. After binding the DNA probe to the gold nanoparticles using a poly A base, ASFV of the complementary sequence is bound. And the reaction is performed according to temperature, volume, and time. Gold nanoparticles were characterized by DLS, TEM, and microplate readers. The detection of ASFV using a gold nanoparticle-DNA probe that shows a convenient and quick reaction can be visually confirmed through colorimet- ric change without on-site equipment, and is an effective tool for early diagnosis of disease.

키워드