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Separation of Staphylococcal Enterotoxin B by Fast Protein Liquid Chromatography
Fast Protein Liquid Chromatography를 이용한 황색포도상구균 장독소 B 분리
- 조아영;
- 김기영;
- 임종국;
- 모창연;
- 문지혜;
- 외 3명
초록
The aim of this study is to develop a method to separate and purify the Staphylococcal enterotoxin B (SEB). The method was based on fast protein liquid chromatography (FPLC) along with gel filtration (GF) after cation exchange chromatography (CIEX) treatment. Various columns such as CM sepharose HP, Cellufine C-500, WorkBeads 40S for CIEX, and GF on Sephacryl S-100 for GF were compared in terms of separation efficiency. Consequently, Work- Beads 40S along with Sephacryl S-100 produced best separation performance. Separated SEB with developed method was verified with ELISA assay. Purified SEB with the method can be used to manufacture rapid detection kit or antibodies.
키워드
staphylococcal enterotoxin B (SEB); fast protein liquid chromatography (FPLC); separation; purification; detection
- 제목
- Separation of Staphylococcal Enterotoxin B by Fast Protein Liquid Chromatography
- 제목 (타언어)
- Fast Protein Liquid Chromatography를 이용한 황색포도상구균 장독소 B 분리
- 저자
- 조아영; 김기영; 임종국; 모창연; 문지혜; 박샛별; 박수희; 엄애선
- 발행일
- 2015-05
- 유형
- Y
- 저널명
- 산업식품공학
- 권
- 19
- 호
- 2
- 페이지
- 111 ~ 116