Effect of dissolved oxygen concentration on nar promoter activity in batch and semi-continuous cultivations

초록

The level of the dissolved oxygen concentration could significantly affect the nar promoter activity in the induction of the gene expression under the anaerobic condition. In batch culture, the β-galactosidase activity was about 12 000 units/min/g cell under the anaerobic condition. The optimum DO concentration for the induction of the gene expression was 0% in both batch and continuous cultivations. In semi-continuous culture, the maximum enzyme activity was about 11 000 units/min/g cell at 0% of the DO concentration. These results indicated that the absolutely anaerobic culture condition was required for the maximum gene expression.

키워드