식이섭취 형태와 일회성 운동이 골격근내 PPAR isoforms 유전자 발현에 미치는 영향

초록

Lim, Y.J., Lee, J.K., Kim, J.K., Moon, H.W., Park, S.H., Lee, J.S. The influence of dietary feeding type and acute exercise on gene expression of PPAR isoforms in skeletal muscle. Exercise Science. 17(4): 401-412, 2008. We determined the interactions of dietary alterations and acute swimming exercise on gene expression of peroxisome proliferator-activated receptor(PPAR) α, β/δ, and γ in red vastus lateralis muscle. Total 32 Sprague-Dawley rats were allocated one of two dietary conditions: either standard chow diet(CHOW, n=16) or high fat diet(FAT, n=16). Rats in each dietary condition were further divided into either exercise(EXE, n=8) or non-exercise (CON, n=8) group. All rats in exercise group were performed 3 hr(30min x 6bouts) acute swim exercise. Major findings of this study were that there was no significant difference in body weight(BW) between CHOW and FAT. There was a significant increase in blood glucose and insulin concentration after 8wk high fat diet compared to standard chow diet. Although high fat diet did not show any significant changes in blood lactate, it significantly increased free fatty acid level at rest(FFA, p<.05). Swimming for 3hr significantly decreased of blood glucose and insulin concentration(p<.05), and significantly increased of lactate and FFA(p<.05). While there was no significant difference in PPARα and β/δ gene expression, PPARγ gene expression was significantly decreased after 8wk high fat feeding. After 3 hr swim exercise, PPARα and β/δ expression was significantly increased(p<.05), but the degree of increase in PPARγ gene expression did not reach statistical significance(p=.059). Based on these results we concluded while high fat feeding predominantly affect in PPARγ gene expression, prolonged acute swim exercise affect mostly of PPARα, and β/δ gene expression. These results suggest that both different types of diet and long lasting acute exercise would have important role in regulating fatty acid metabolism, but these isoforms would also have unresolved unique cellular signaling cascade to exert their independent roles in skeletal muscle.

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