Therapeutic effects of CTLA4Ig-overexpressing mesenchymal stem cell-derived extracellular vesicles in a mouse model of rheumatoid arthritis

초록

BackgroundRheumatoid arthritis (RA) is a chronic autoimmune disease characterized by cartilage damage and bone erosion. Current pharmacological treatments often fail to repair damaged tissues and may cause severe immune-related side effects. Moreover, some patients exhibit inadequate responses to existing therapies. This study evaluated the therapeutic potential of extracellular vesicles (EV) derived from immortalized mesenchymal stem cells (iMSCs) overexpressing cytotoxic T lymphocyte-associated antigen-4 immunoglobulin fusion protein (CTLA4Ig) (CT-EV) compared with iMSC-derived EVs (ASC-EV).MethodsFollowing EV characterization and in vitro functional assessments, collagen-induced arthritis (CIA) model mice (n = 10/group) were treated with Dulbecco's phosphate-buffered saline (dPBS, 150 mu L, twice weekly; Group C), ASC-EV (derived from the culture supernatant of 2 x 106 iMSCs/150 mu L of dPBS, twice weekly; Group E), CT-EV (derived from the culture supernatant of 2 x 106 CTLA4Ig-overexpressing iMSCs/150 mu L of dPBS, twice weekly; Group CT), or methotrexate (3 mg/kg, three times per week; Group M). A normal control group received dPBS (150 mu L, twice weekly; Group N).ResultsCT-EV showed a significant increase in EV quantity and the production of CTLA4, transforming growth factor beta 1, and interleukin (IL)-1 receptor antagonist compared with ASC-EV. In mitogen-stimulated immune cells from CIA mice, CT-EV significantly reduced IL-6, IL-10, and Regulated upon Activation, Normal T cell Expressed and Presumably Secreted (RANTES) levels. Administration of both ASC-EV and CT-EV led to a decrease in macrophage proportions and an increase in T helper type 2 cells and serum IL-4 levels. Furthermore, CT-EV treatment resulted in additional reductions in anti-CII antibody levels, C-telopeptide II concentrations, and the proportion of CD138(+) cells, thereby contributing to cartilage protection.ConclusionsCT-EV demonstrated superior therapeutic effects compared with ASC-EV in the CIA model, highlighting its potential as an effective treatment strategy for RA.

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