Addition and measurement of water in carbon dioxide mobile phase for supercritical fluid chromatography

초록

A method for the addition of water to supercritical CO<inf>2</inf> is described. Carbon dioxide, the most widely used mobile phase in supercritical fluid chromatography, is a relatively non-polar fluid, and hence the addition of small amounts of polar modifiers could be necessary to migrate polar solutes. In this work, supercritical CO<inf>2</inf> is delivered from the pump to a μ-Porasil column that is saturated with water. After passing through the μ-Porasil column, supercritical CO<inf>2</inf> is changed to a new mobile phase with different polarity, and it is possible to separate polar samples by using this new mobile phase. The amount of water dissolved in supercritical CO<inf>2</inf> is measured by an amperometric microsensor, which is prepared from a thin film of perfluorosulfonate ionomer.

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