Casuarinin suppresses TARC/CCL17 and MDC/CCL22 production via blockade of NF-κB and STAT1 activation in HaCaT cells

초록

Casuarinin is a naturally occurring tannin that is isolated from the leaves of Hippophae rhamnoides. It has been shown to have anti-oxidant, anti-cancer, anti-viral, and anti-inflammatory activities. The aim of this study was to investigate the possible mechanism by which casuarinin inhibits TNF-alpha/IFN-gamma-induced Th2 chemokines expression in the human keratinocytes cell line HaCaT. We found that casuarinin suppressed TNF-alpha/IFN-gamma-induced expression of TARC and MDC mRNA and protein in HaCaT cells. Casuarinin significantly inhibited TNF-alpha/IFN-gamma-induced activation of NF-kappa B, STAT1, and p38 MAPK. Furthermore, we observed that p38 MAPK contributes to inhibition of TNF-alpha/IFN-gamma-induced TARC and MDC production by blocking NF-kappa B and STAT1 activation in HaCaT cells. Taken together, these results suggest that casuarinin may exert anti-inflammatory responses by suppressing TNF-alpha/IFN-gamma-induced expression of TARC and MDC via blockage of p38 MAPK activation and subsequent activation of NF-kappa B and STAT1. We propose that it could therefore be used as a therapeutic agent against inflammatory skin diseases. (C) 2011 Elsevier Inc. All rights reserved.

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