The immunomodulatory activities of a thermostable galacto-mannanase and their impact on the performance of weaned piglets

초록

The objective of this study was to evaluate the immunomodulatory impact of thermostable galacto-mannanase on the performance of weaned pigs. The experiment involved 320 mixed-sex pigs, weaned at 3 weeks of age, with an average body weight of 5.97 +/- 1.06 kg. The pigs were randomly assigned to 4 treatments based on their body weight (BW). The randomization was conducted 10 times, with each replicate containing 8 piglets. The experiment lasted for 6 weeks (phase 1, d 1-21; phase 2, d 22-42), with the pigs assigned to one of the four dietary groups: Corn-soybean-meal (SBM) basal diet as control (CON), CON+ 0.01 % mannanase (MAN) as MAN1, CON+ 0.02 % MAN (MAN2), and CON+ 0.03 % MAN (MAN3). The results showed that the final BW was higher (p <0.05) in MAN2 and MAN3 compared with MAN1, and MAN1 was higher (p < 0.05) than CON. In phase 1, the average daily gain (ADG) and feed efficiency (G/F) were higher (p < 0.05) in MAN2 and MAN3 compared with CON, while in phase 2 and the overall phase, the ADG and G/F were higher (p < 0.05) in the MAN-supplemented diets compared with CON. The apparent total tract digestibility coefficient (CATTD) of dry matter, gross energy, nonstarch polysaccharide, and mannan were higher (p < 0.05) in MAN2 and MAN3 in phase 1 and phase 2 respectively. The intestinal viscosity was lower (p <0.05) as the level of MAN increased from MAN1 through MAN2 to MAN3. The ileal and caecal Escherichia coli was lower (p <0.05) in MAN2 and MAN3 compared with CON. The duodenal villus height (VH) and VH/crypt depth (CD) were higher (p < 0.05) in MAN2 and MAN3 compared with CON. The jejunal VH was higher (p < 0.05) in the MAN-supplemented diets, while the jejunal VH/CD was higher (p < 0.05) in the MAN2 and MAN3 compared with CON. The ileal VH was higher (p < 0.05) in the MAN2 and MAN3 compared with CON, while ileal VH/CD was higher (p < 0.05) in all the MANsupplemented diets. The insulin and glucose were higher (p < 0.05) in MAN2 and MAN3 in phase 1, while in phase 2, the immunoglobulin M was higher (p < 0.05) in MAN2 and MAN3 compared with CON. The insulin was higher (p < 0.05) in MAN3 compared with CON, while glucose was higher (p < 0.05) in all MAN-supplemented diets. In conclusion, supplementing MAN at increasing levels of 0.01-0.03 % could enhance growth performance in weaned pigs. This improvement may be attributed to MAN's ability to reduce E. colt and intestinal viscosity, which in turn enhanced blood insulin, glucose, VH, VH/CD, and ATTD of nutrients.

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