Expression Patterns of an Abiotic Stress-inducible Dehydrin Gene, LeDhnT14, in Tomato

초록

A full length cDNA encoding Lycopersicum esculentum dehydrin Tas14 (designated as LeDhnT14) was isolated and investigated for the expression pattern of dehydrin Tas14 gene in tomato plant under different abiotic stresses. Phylogenetic analysis based on the deduced amino acid sequence of the dehydrin Tas14 gene (LeDhnT14) cDNA from tomato revealed a high sequence similarity to other dehydrin genes. Southern blot showed that LeDhnT14 is a multiple copy gene in the tomato genome. The organ specific expression profiling indicated that LeDhnT14 was expressed in mature leaves, stem, and flowers, but with no detectable expression in root. Northern blot analysis revealed that various environmental stresses such as salt, drought, coldness, MV, ABA, and H2O2 induced significant expression of LeDhnT14. These results indicated that the LeDhnT14 plays an important role in tomato responses to abiotic stresses and may be useful in improving of plant tolerance to abiotic stresses.

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