In vitro evaluation of nickel nanoparticles against various pathogenic Fusarium species

초록

The objective of this study was to evaluate antifungal activity of nickel nanoparticles against Fusarium species as an alternative to existing methods. In this study, nickel nanoparticles at concentrations of 10, 20, 50 and 100 ppm, were evaluated for their antifungal activity on42 isolates of Fusarium belonging to different species isolated from crop field soils of different locations in Korea. The fungal isolates were grown on three different media, potato dextrose agar, corn meal agar and malt extract agar, amended with nickel nanoparticles. The results indicate that nickel nanoparticles at concentrations of 50 and 100 ppm inhibited the mycelial growth of Fusarium isolates investigated. Nickel nanoparticles at a concentration of 100 ppm caused more than 90% inhibition of mycelial growth in six isolates on malt extract agar media. The range of growth inhibition was 24.7-90.2% and 21.67-85.1% at a concentration of 100 ppm on corn meal agar and potato dextrose media, respectively. The light and scanning electronic microscope examinations revealed that the nickel nanoparticles caused damage of mycelia and spores of tested Fusarium species. This study suggests that nickel nanoparticles at high concentration could be used to control Fusarium fungi. However, further studies are needed to assess the effect of nickel nanoparticles on the growth of host plant. © 2016, Sphinx Knowledge House. All rights reserved.

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