Adeno-Associated Viral Vector-Mediated Interleukin 10 Gene Transfer in Sprague-Dawley Rat Skin Allograft

초록

Background. Viral vector-mediated interleukin 10 gene transfer is one of the most promising methods for the prevention of graft rejection. Adeno-associated virus (AAV) is a nonpathogenic helper-dependent parvovirus. This is one of the most promising vehicles for gene delivery. Our study aimed to analyze the immune-suppressive potential of recombinant adeno-associated virus-mediated rat IL-10 in rat skin allograft. Methods. Dorsal skin from Sprague-Dawley rats was grafted to Fischer 344 rats. In vivo AAV-viral IL-10 (vIL-10) gene transfer was done in the experimental group by direct subcutaneous injection. Observation of graft appearance, cytologic and immunohistochemical testing, and confocal immunofluorescence were performed at postoperative days 5 and 10. Results. The viral IL-10 gene-treated group showed improved graft survival and reduced acute rejection response compared to the control group. IL-10 expression on skin was identified by immunohistochemistry and confocal microscopy. Conclusion. AAV IL-10 gene transfer could be an effective immunosuppressive method for preventing skin allograft rejection.

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