Eugenol Inhibits Excitotoxins-Induced Delayed Neurotoxicity,Oxidative Injury and Convulsion

초록

In previous our studies, we have reported that eugenol derived from Eugenia caryophyllata (Myrtaceace) exhibits acute N-methyl-D-aspartate (NMDA)- and oxygen/glucose deprivation-induced neurotoxicity in primary cortical cultures and protects hippocampal neurons from global ischemia. In this study, we investigated whether the extracts and fractions of E. caryophyllata or eugenol shows the neuroprotective effects against delayed neuronal injury evoked by NMDA or a-amino-3-hydroxy-5-methylisoxazole propionate (AMPA), and oxidative damage induced by arachidonic acid-, hydrogen peroxide-, FeCl2/ascorbic acid-, and buthionine sulfoximine (BSO) in primary cortical cultures. We examined the neurotoxicity of eugenol itself in cultures and inhibitory effect of eugenol on NMDA- or kainate (KA)-induced convulsion in BALB/c mice. Each water, methanol extract and methanol fraction of E. caryophyllata was significantly attenuated NMDA-induced delayed neurotoxicity, respectively. Eugenol exhibited a significant inhibitory action against the convulsion evoked by NMDA and KA, and reduced delayed or brief neurotoxicity induced by NMDA, AMPA, and various oxidative injuries. These results suggest that eugenol derived from E. caryophyllata may contribute the neuroprotection against delayed-type excitotoxicity and excitotoxins-mediated convulsion through the amelioration of oxidative stress.

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