Complete nucleotide sequence and genome organization of a dsRNA partitivirus infecting Pleurotus ostreatus

초록

The nucleotide sequences of the genomic dsRNA mycovirus infecting Pleurotus ostreatus (P.ostreatus virus 1; PoV 1) were determined and compared to the sequences of the other mycoviruses belonging to partitiviruses and totivirues. PoV 1 dsRNA-1 and dsRNA-2 had genomes of 2296 and 2223 nucleotides, respectively. The purified virus preparations contained isometric particles of 28-30 run in diameter, and also the same two dsRNAs were isolated from purified virus preparations. The sequences of PoV 1 dsRNA-1 and dsRNA-2 had GC contents of 48.4 and 51.5%, respectively. dsRNA-1 had 78 and 97 nucleotides of 5'- and 3'-untranslated region (UTR) while dsRNA-2 had 114 and 198 nucleotides of 5- and 3'-UTR, respectively. Computer analysis of putative open reading frame (ORF) shows that dsRNA-1 and dsRNA-2 contain a single ORF encoding proteins of 82.2 and 71.1 kDa that show high sequence identity with RNA-dependent RNA polymerase and capsid protein of partitiviruses, respectively. When compared to other dsRNA mycoviruses in a phylogenetic analysis they were found to form a distinct virus clade with partitiviruses, and were more distantly related to totiviruses. (C) 2004 Elsevier B.V. All rights reserved.

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