헛개나무의 체세포배발생 및 식물체 재분화

초록

An efficient and reproducible procedure for the large scale propagation of Hovenia dulcis Thunb. is described. Shoot primodia emerging from the leaf surface was induced from MS medium supplemented with NAA. Stem cuttings were suitable explants for multiple shoot proliferation. They produced axillary shoots which branched repeatedly, yielding an average of 7 shoots per explants after 4 weeks in culture, when cultured on a woody plant medium (WPM) containing 0.1㎎/ℓ BA and 0.1㎎/ℓ NAA. Stem, leaf and root segments from axenic seedlings were used as explant source to induce somatic embryogenesis. A high frequency of somatic embryos were induced directly from leaf in MS medium with NAA, 2,4-D and in medium containing NAA, 2,4-D with BA. Somatic embryos were germinated in MS medium supplemented with 1㎎/ℓ GA3. Somatic embryos proliferated secondary somatic embryos rapidly after transfer to MS medium supplemented with 1㎎/ℓ kinetin, 1㎎/ℓ GA3 and 2% dextrose.

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