Effects of a mixture of Centella asiatica and Portulaca oleracea extract on expression of growth factors and osteogenic activity in an in vitro system; 병풀과 쇠비름 복합물이 in vitro 체계에서 성장인자 발현 및 조골활성에 미치는 영향

초록

Purpose: A mixture of Centella asiatica extract and Portulaca oleracea extracts (FSG-CA) was prepared in a 1:1 ratio. There have been no studies on the health benefits of this mixture to date. This in vitro study aimed to investigate the efficacy of FSG-CA in increasing the growth in height. Methods: HepG2 hepatoma cells were treated with various concentrations (0, 1, 5, 10, or 20 μg/mL) of FSG-CA. In HepG2 cells, the protein and mRNA expressions of insulin-like growth factor-1 (IGF-1) and IGF-binding protein-3 (IGFBP-3)- were measured by enzyme-linked immunosorbent assay (ELISA) and quantitative reverse-transcription polymerase chain reaction (RT-PCR), respectively. MC3T3-E1 osteoblastic cells were incubated in an osteoblast differentiation-inducing medium and treated with various concentrations (0, 25, 50, 100, or 200 μg/mL) of FSG-CA. In MC3T3-E1 cells, alkaline phosphatase (ALP) activity was assayed. Mineralization was determined by staining the cells with alizarin red S. In addition, the mRNA expressions of collagen type I alpha 1 chain (COL1A1), and bone morphogenetic protein-2 (BMP-2) were measured by quantitative RT-PCR. Results: In HepG2 cells, FSG-CA increased the production of IGF-1 and IGFBP-3. The mRNA expressions of IGF-1 and IGFBP-3 were increased in the cells treated with FSG-CA. In MC3T3-E1 cells, FSG-CA increased ALP activity, an early-stage marker of osteoblast differentiation, and mineralization, a late-stage marker of osteoblast differentiation. In addition, the mRNA expressions of COL1A1 and BMP-2 were increased in the cells treated with FSG-CA. Conclusion: These results demonstrate that FSG-CA can increase the expression of IGF-1 and IGFBP-3, growth regulators secreted by the liver, in HepG2 cells and osteoblast activity, a key action in longitudinal bone growth, in MC3T3-E1 cells. This suggests the potential of FSG-CA as a functional food ingredient for promoting growth in height. © 2025 The Korean Nutrition Society.

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