Assessment of chromium-contaminated groundwater using a thiosulfate-oxidizing bacteria (TOB) biosensor

초록

The effect of Cr6+-contaminated groundwater was assessed using thiosulfate-oxidizing bacteria (TOB). Electrical conductivity (EC), pH, and sulfate production were determined based on thiosulfate oxidation. Final pH values in the different test treatments of Cr6+-contaminated groundwater (50-1000 mu g Cr6+ L-1) ranged from 2.02 +/- 0.09 to 7.76 +/- 0.07 and EC ranged from 5.95 +/- 0.03 to 3.63 +/- 0.03 mS cm(-1). Inhibition of TOB due to Cr6+ was between 16.7% and 100%, with higher levels of inhibition occurring at higher Cr6+ concentrations. The median effective concentration (EC50) was 78.96 mu g Cr6+ L-1. These data demonstrate that TUB can detect less than 100 mu g L-1 of Cr6+ in the groundwater and can be used as an effective bio-assay for toxicity assessment. (C) 2013 Elsevier Ltd. All rights reserved.

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