Polysaccharide isolated from the liquid culture broth of Inonotus obliquus suppresses invasion of B16-F10 melanoma cells via AKT/NF-κB signaling pathway

초록

A number of polysaccharides exhibit pharmacological activities. Polysaccharides derived from Inonotus obliquus (PLIO) appear to have various potential pharmacological properties, including anti-tumor activity. However, the molecular mechanisms underlying these properties remain to be elucidated. The present study investigated the anti-metastatic potential of PLIO and the underlying signaling pathways in B16-F10 murine melanoma cells using the MTT colorimetric assay, in vitro migration and invasion assays, and flow cytometric and western blot analyses. PLIO inhibited the invasion of B16-F10 cells and suppressed the expression of matrix metalloproteinases. PLIO treatment inhibited nuclear factor-kappa B (NF-kappa B) nuclear translocation in B16-F10 cells. In addition, PLIO treatment inhibited the phosphorylation of c-Jun N-terminal kinases and AKT. These results suggest that PLIO may suppress the invasion of highly metastatic melanoma cells via inhibition of the AKT/NF-kappa B signaling pathways.

키워드