Genetic diversity and relationships among rice accessions (Oryza sativa L.) of cultivated and weedy types using CACTA-TD and AFLP markers

초록

For understanding the genetic diversity and genetic relationship between cultivated and weedy types, we evaluated genetic variation of 80 accessions of rice (O. sativa). This included 42 cultivated accessions and 38 weedy accessions with the help of AFLP and CACTA-TD. A total of 542 loci were analyzed (255 for AFLP and 287 for CACTA-TD) of which AFLP markers exhibited 75% of polymorphism and transposon based CACTA-TD markers exhibited 93% of polymorphism. The average genetic diversity value for all 80 accessions using AFLP markers was 0.226(Cultivated - 0.210; Weedy 0.241) and based on CACTA-TD markers was 0.281 (Cultivated - 0.294; Weedy 0.269). A UPGMA phylogenetic tree revealed three major groups for both the marker system. The average polymorphic content value obtained with AFLP and CACTA-TD markers were 0.21 and 0.232, Effective multiplex ratio (AFLP - 47.50; CACTA-TD- 66.75), Marker Index (AFLP - 9.94; CACTA-TD- 21.13) and Resolving power (AFLP - 19.53; CACTA-TD- 34.62) indicated that the CACTA-TD markers were relatively efficient than AFLP markers.

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