Chiral separation of 2,4-dinitrophenyl amino acids using 3-O-methyl-&beta-cyclodextrin-bonded stationary phase in reversed-phase liquid chromatography

초록

Reversed-phase liquid chromatography (RPLC) separation factors for racemic 2,4-dinitrophenyl (DNP) amino acids (Phe and Trp) having various substituents at different positions were measured on native β-CD and heptakis(3-O-methyl)-β-CD as the chiral stationary phase in order to compare enantioselectivity of these phases. Both native β-CD and heptakis(3-O-methyl)-β-CD showed good enantioselectivity for the DNP-amino acids investigated. Enantioselectivities of the two CD phases for the DNP-amino acids vary with the type and position of the substituent on the DNP moiety of the amino acids. Heptakis(3-O-methyl)-β-CD, the cavity of which is more electron-rich than that of native β-CD, showed in general much better enantioselectivity for the amino acid derivatives studied. Copyright (C) 1998 Elsevier Science B.V.

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