Complete genome sequences of three isolates of Bean yellow mosaic virus identified from freesia (Freesia refracta Klatt.)

초록

We collected leaf samples of freesia displaying mild yellow mosaic symptoms from two freesia cultivars referred to as Pinksia-1 and Pinksia-2 to identify viruses infecting freesia (Freesia refracta Klatt.). By RNA-sequencing and bioinformatic analyses, we identified three complete genomes of Bean yellow mosaic virus (BYMV) in the genus Potyvirus belonging to the family Potyviridae. The genome size of all three BYMV isolates was 9,528 nucleotides, and the nucleotide identity of the three BYMV genomes ranged from 99.90% to 99.91%. All three isolates in this study were closely associated with the known BYMV isolate Fr from freesia in Korea. The genome of BYMV was composed of positive-sense single-stranded RNA and encoded a large polyprotein, which was further self-cleaved into 10 different mature viral proteins and the pretty interesting potyviridae ORF (PIPO) protein. Taken together, we report the complete genomes of three BYMV isolates identified from freesia in Korea by RNA-sequencing.

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