Phyto-fabrication of biocompatible silver nanoparticles using Potentilla chinensis Ser leaves: characterization and evaluation of its antibacterial activity

초록

The present study designed to fabricate the silver nanoparticles from the aqueous leaf extracts of Potentilla chinensis Ser ( Pc-AgNPs) and their physicochemical characterization were affirmed by spectroscopic and analytical techniques. The absorption maxima at 430 nm verified the synthesis of Pc-AgNPs. Further, the XRD spectroscopy authenticated the crystal, face-centered cubic (fcc) structure of Pc-AgNPs with the 2. value of 38.13 degrees, 44.34 degrees, 64.31 degrees and 77.36 degrees. FT-IR spectrum revealed the contribution of functional biomolecules in reduction of Ag+ to Ag-0. Further the zeta potential, particle size and polydispersity index (PDI) of Pc-AgNPs were found as -36.6 +/- 0.52 mV, 136 +/- 24.82 nm and 0.202, respectively. Besides, the anisotropic structure of Pc-AgNPs with an average size of 24.3 +/- 4.6 nm was confirmed by TEM analysis. In vitro antibacterial action of Pc-AgNPs was explored by agar well diffusion, then microtiter method were performed to detect the MIC and MBC of Pc-AgNPs. Further, the cellular changes in bacterial cells were visualized by confocal laser scanning and scanning electron microscopy. Pc-AgNPs considerably inhibited the growth of Salmonella enterica, Staphylococcus aureus, Bacillus cereus and Escherichia coli. The minimum inhibitory concentration (MIC) of Pc-AgNPs was found for S. aureus (5.0 +/- 1.15 mu g/mL), B. cereus (6.25 +/- 0.58 mu g/mL), S. enterica (3.75 +/- 0.73 mu g/mL) and E. coli (3.12 +/- 1.25 mu g/mL). Besides the biocompatible nature of Pc-AgNPs was explored in HEK-293 cell line, which revealed that up to 500 mu g/mL did not cause any significant toxicity. Taken together, our results conclude that Pc-AgNPs have the potential antibacterial activity with promising biocompatibility in HEK-293 cells.

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