Development of a Multiplex Loop-Mediated Isothermal Amplification (LAMP) Assay for On-Site Diagnosis of SARS CoV-2

Sik Jang, Woong; Lim, Da-hye; Yoon, Jung; Kim, Ahran; Lim, Minsup; Nam, Jeonghun; Yanagihara, Richardt T.; Ryu, Sookwon; Jung, Bokyeung; Ryoo, Namhee; Lim, Chae-seung

doi:10.1201/9781003298038-40

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Development of a Multiplex Loop-Mediated Isothermal Amplification (LAMP) Assay for On-Site Diagnosis of SARS CoV-2

Sik Jang, Woong;
Lim, Da-hye;
Yoon, Jung;
Kim, Ahran;
Lim, Minsup;
... Ryu, Sookwon;
외 5명

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초록

Loop-mediated isothermal amplification is a highly sensitive, low-cost, single-tube technology to detect the target nucleic acid sequences. LAMP has been widely applied to detect various microbial pathogens. In particular, reverse-transcription LAMP has been used for point-of-care-testing for RNA virus infections. In addition, when conducting clinical tests in large quantities, the number of clinical trials more than doubles, and the advantage of a rapid diagnosis of the LAMP assay may be diluted. Since the multiplex SARS CoV-2 RdRP/N/IC RT-LAMP assay takes less time, compared to the commercial Allplex 2019-nCoV Assay and PowerChek 2019-nCoV Real-time PCR kit, it shows promise for deployment as an on-site molecular diagnostic test. © 2023 Jenny Stanford Publishing Pte. Ltd.

키워드

adenovirus (AdV); clinical performance; Coronavirus Disease 2019 (COVID-19); cross-reactivity; human bocavirus (HboV); human enterovirus (HEV); human rhinovirus (HRV); Influenza (Inf); institutional review board (IRB); limits of detection (LOD); loop-mediated isothermal amplification (LAMP); molecular diagnotics; nasopharyngeal (NP); oropharyngeal (OP); parainfluenza virus (PIV); point-of-care (POC); polymerase chain reaction (PCR); quantitative polymerase chain reaction (qPCR); quantitative reverse transcription polymerase chain reaction (RT-qPCR); respiratory syncytial virus (RSV); reverse-transcription loop-mediated isothermal amplification (RT-LAMP); RNA-dependent RNA polymerase (RdRP); Severe Acute Respiratory Syndrome Coronavirus 2 (SARS CoV-2); World Health Organization (WHO)

제목: Development of a Multiplex Loop-Mediated Isothermal Amplification (LAMP) Assay for On-Site Diagnosis of SARS CoV-2

저자: Sik Jang, Woong; Lim, Da-hye; Yoon, Jung; Kim, Ahran; Lim, Minsup; Nam, Jeonghun; Yanagihara, Richardt T.; Ryu, Sookwon; Jung, Bokyeung; Ryoo, Namhee; Lim, Chae-seung

DOI: 10.1201/9781003298038-40

발행일: 2023

유형: Book chapter

저널명: Advances in Medical Imaging, Detection, and Diagnosis

페이지: 1081 ~ 1098

언어: ENG

출판사: Jenny Stanford Publishing

분량: 18 페이지