CACTA transposon-derived Ti-SCARs for cultivar fingerprinting in rapeseed

초록

Transposon display (TD) is a technique for analyzing polymorphic insertions and excisions of transposable elements. We used the CACTA transposon to develop cultivar-specific transposon insertion-sequence characterized amplified regions (Ti-SCARs) for rapeseed (Brassica napus). Using 16 combinations of TD primers, we detected 19 cultivar-specific fragments among six rapeseed cultivars. Of the 16 primer pairs, 12 successfully amplified targets in B. napus and six amplified novel, cultivar-specific markers. Cultivar-specific markers can be used for cultivar fingerprinting and marker-assisted selection in the rapeseed breeding program. Moreover, because Ti-SCARs are based on transposon insertions, Ti-SCAR markers may be used in reverse genetic techniques for isolating novel genes in plants.

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