발효차중의 미량 성분인 gallic acid 산화물 purpurogallin carboxylic acid의 항염증 효과

초록

The principal objective of the current study was to isolate a purpurogallin derivative as an oxidation product from gallic acid, in an effort to assess the anti-inflammatory effects of this compound. Purpurogallin derivative is known to be the one of the oxidation products of gallic acid. This compound has been identified as a minor chemical component in fermented tea products. It has been previously demonstrated that theaflavins, the oxidation products of catechins found in fermented tea products, exert profound antioxidant and anti-inflammatory effects. However, the biological activities of a minor chemical component in fermented teas have yet to be evaluated. Purpurogallin carboxylic acid (PCA) was identified as a major oxidation product of gallic acid from a peroxidase/hydrogen peroxide oxidation model system. The identity of the PCA was verified by ¹H NMR, ¹³C NMR and MS techniques. PCA treatment significantly suppressed the generation of pro-inflammatory mediators including nitric oxide and IL-6 in lipopolysaccharide (LPS)-stimulated RAW264.7 murine macrophages. According to the nitrite assay, PCA 100, 75, and 50 μg/mL treatment dose-dependently inhibited NO production by 57.6, 41.5, and 21.8%, respectively, in LPS-stimulated RAW264.7 murine macrophage cells. Moreover, IL- 6 production was inhibited to a significant degree with PCA treatment of 100 and 75 μg/mL at 43.1 and 23.9%, respectively. PCA treatment also significantly suppressed PGE₂ production at levels of 100 and 75 μg/mL. These results showed that PCA exerts inhibitory effects on the production of inflammatory mediators.

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