Antioxidant and Anti-proliferative Activities of Different Solvent Fractions from Methanol Extracts of Sparganiun stoloniferum

초록

The antioxidant and antiproliferation effects of fractions from methanol extract of Sparganiun stoloniferum were investigated in this study. The ethyl acetate fraction of the methanol extract showed significant antioxidant activity against alpha,alpha-diphenyl-picrylhydrazyl (DPPH) and hydroxyl (center dot OH) radical scavenging, and it showed greater reducing power than the n-hexane, dichloromethane, n-butanol and water fractions. Qualitative HPLC analysis was performed on the extractions. The high content of phenolics in ethyl acetate fractions were believed to be responsible for its antioxidant activity, which was the strongest in all five fractions. Moreover, the ethyl acetate fraction showed a dose-dependent inhibition of cell proliferation with an EC50 of 139.21 +/- 0.54 mu g.mL(-1) against the human colon cell, HT-29. The anti-proliferative effects of the ethyl acetate fraction were associated with apoptosis. Semi-quantitative RT-PCR analysis indicates that, after treatment with the ethyl acetate fraction, the expression of caspase-3, p53, and Box in HT-29 cells was significantly up-regulated and c-myc expression was induced. These findings demonstrate that the ethyl acetate fraction of S. stoloniferum has potent antioxidant and antiproliferation effects and induces apoptosis. Thus, the extract of S. stoloniferum may be considered as a potential functional food resource and complementary medicinal supplement.

키워드