Detection of Rhizina undulata in Soil by Nested-PCR Using rDNA ITS-specific Primer

초록

Rhizina undulata is the fungus, which causes Rhizina root rot on coniferous trees. Nested-PCR using ITS-specific primer was applied to detect R. undulata from the soils of Japanese blackpine (Pinus thunbergii) forests infested with the disease in Seocheon, Chungnam Province, SouthR.undulata were present, dead trees only present, fruit bodies only present, and both were absent.Nested-PCR products specific to R. undulata ITS-region were amplified. Positive reactions were foundin some samples from the sites, where dead tres and fruit bodies of R. undulata were absent as welas where both of those were present. R. undulata was mainly detected in the soil samples from thedepth of 5~20 cm under the soil surface. These results show that the nested-PCR could be used toR. undulata in the soils of pine forests.Key.words:Rhizina undulata, ITS-specific primer, nested-PCR