Molecular Phylogenetic Classification of Dermatophytes Isolated from Dogs and Cats

초록

Using internal transcribed spacer 1 (ITS1) region ribosomal DNA sequences from 9 strains of Microsporumcanis and 5 strains of Microsporum gypseum isolated from dogs and a cat with dermatophytosis, we demonstratedthe mutual phylogenetic relationship of these strains. Nucleotide sequence analysis of the ITS1 gene fragments fromthe 9 strains of M canis had the 100% nucleotide sequence similarities and the 5 strains of M gypseum also hadthe 100% nucleotide sequence similarities. The phylogenetic analysis of the nucleotide sequences of the 9 strains ofM canis formed a nested cluster with the reference strains of M canisEurope. M canis were genetically distinct from the other reference strains of Microsporum spp, but M distortum, Mequinum, and M ferrugineum were genetically very close to M canis. M gypseum form a cluster in the phylogenetictree with M canis as an outgroup. The molecular analysis of ITS1 genes provided the useful information for theidentification of these Microsporum species and the understanding of their relationship.

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