Sensitive and specific detection of Xanthomonas campestris pv. vesicatoria by PCR using pathovar-specific primers based on rhs family gene sequences

Park, Dong Suk; Shim, Jae Kyung; Kim, Jung Sun; Lim, Chun Keun; Shrestha, Rosemary; Hahn, Jang Ho; Kim, Hong Gi

doi:10.1016/j.micres.2006.11.005

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Sensitive and specific detection of Xanthomonas campestris pv. vesicatoria by PCR using pathovar-specific primers based on rhs family gene sequences

Park, Dong Suk;
Shim, Jae Kyung;
Kim, Jung Sun;
Lim, Chun Keun;
Shrestha, Rosemary;
외 2명

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초록

The present study describes PCR assay to detect bacterial. spot caused by Xanthomonas campestris pv. vesicatoria in pepper and tomato. One set of PCR primer was developed to amplify gene required for an rhs family gene homologous to rhsA, cell envelope biogenesis, outer membrane. Only a PCR product of a 517 bp was produced in PCR reaction with the Xanthomonas campestris pv. vesicatoria (XCVF/XCVR) primer set. A specific, and highly sensitive and rapid PCR assay for the detection of X. campestris pv. vesicatoria was achieved. The protocol can be used as a reliable diagnostic tool. for specific detection of X. campestris pv. vesicatoria in pepper or tomato. (C) 2007 Elsevier GmbH. All rights reserved.

키워드

Xanthomonas campestris pv. vesicatoria; Bacterial spot; Pepper; Tomato; Detection; PLANT-PATHOGENIC BACTERIA; DNA-SEQUENCES; IDENTIFICATION; STRAINS; AMPLIFICATION; PEPPER; CITRI; RICE

제목: Sensitive and specific detection of Xanthomonas campestris pv. vesicatoria by PCR using pathovar-specific primers based on rhs family gene sequences

저자: Park, Dong Suk; Shim, Jae Kyung; Kim, Jung Sun; Lim, Chun Keun; Shrestha, Rosemary; Hahn, Jang Ho; Kim, Hong Gi

DOI: 10.1016/j.micres.2006.11.005

발행일: 2009

유형: Article

저널명: Microbiological Research

권: 164

호: 1

페이지: 36 ~ 42