Improved viability of freeze-thawed embryonic stem cells after exposure to glutathione

Kim, Gil Ah; Lee, Seung Tae; Ahn, Ji Yeon; Park, Jong Heum; Lim, Jeong Mook

doi:10.1016/j.fertnstert.2010.01.073

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Improved viability of freeze-thawed embryonic stem cells after exposure to glutathione

Kim, Gil Ah;
Lee, Seung Tae;
Ahn, Ji Yeon;
Park, Jong Heum;
Lim, Jeong Mook

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WEB OF SCIENCE

13

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SCOPUS

12

초록

Adding a potent antioxidant, glutathione (GSH), to a cryoprotective solution consisting of dimethyl sulfoxide and ethylene glycol and/or postthaw culture medium significantly improved the postthaw viability of mouse embryonic stem cells. This effect, which was caused by a decrease in reactive oxygen species, was only induced by exposure of embryonic stem cells during cryopreservation. (Fertil Steril (R) 2010;94:2409-12. (C)2010 by American Society for Reproductive Medicine.)

키워드

Stem cells; cryopreservation; antioxidant; reactive oxygen species; OXIDATIVE STRESS; CRYOPRESERVATION METHOD; ROCK INHIBITOR; IN-VITRO; VITRIFICATION; MITOCHONDRIAL; TOLERANCE; EFFICIENT; PROLIFERATION; ESTABLISHMENT

제목: Improved viability of freeze-thawed embryonic stem cells after exposure to glutathione

저자: Kim, Gil Ah; Lee, Seung Tae; Ahn, Ji Yeon; Park, Jong Heum; Lim, Jeong Mook

DOI: 10.1016/j.fertnstert.2010.01.073

발행일: 2010-11

유형: Editorial Material

저널명: Fertility and Sterility

권: 94

호: 6

페이지: 2409 ~ 2412