Semen Characteristics in Bulls Produced by Embryo Transfer in Hanwoo (Korean native cattle) of Hoengseong Area

초록

The aim of this study was to investigate the individual difference of semen characteristics in bulls produced by embryo transfer (ET) in Hanwoo. A total of 5 bulls (Bull No. 0846, 3010, 8777, 8778 and 8807) which had been produced by ET were examined. Semen was collected with artificial vagina method and were replicated three to four times for each bulls. Extended semen was evaluated survival rate, mitocondrial activity, abnormal spermatozoa and stability of the sperm membrane. Acrosome reaction and acrosome status of sperm was measured by coomassie brilliant blue (CBB) staining and chlortetracycline (CTC) assay, respectively. As a result, the rates of survival in semen of bull No. 0846, 3010 and 8777 were significantly (p<0.05) higher than that of bull No. 8778 and 8807. Acrosome reaction rate in bull No. 8778 was significantly lower (p<0.05) than that of the other semens, but membrane stability was not different among the semen of bull No. 0846, 3010 and 8777. Also the mitocondrial function, acrosome status and morphological abnormality were not significantly different among bulls. Therefore, bull No. 0846 can be consider as the highest reproductive ability male animal on basis of semen characteristics.

키워드